Prof. Dirk Bumann, Principle Investigator for BattleX - Biozentrum, Basel
Field of research
- System-level analysis of metabolic host
- Pathogen interactions in a typhoid fever model - Development of novel antimicrobials
- Rational vaccine development for intracellular pathogens
Specific Contribution to Research in BattleX
Using large-scale Shigella mutation and chemical inhibitors for human and Shigella enzymes to modulate intracellular growth of this pathogen in human cells.
The lab has reconstructed the Salmonella metabolism network during infection based on a comprehensive analysis of Salmonella activities in infected mice. It combines genome-scale in silico modeling, quantitative proteomics, and mutant phenotyping to obtain a quantitative understanding of metabolite transfer between host cells and intracellular Salmonella. This will enable comparison with data generated in this project on Shigella, which are closely related to Salmonella but reside in a distinct intracellular niche.
Lab Members Involved in BattleX
Dr David Kentner, Postdoctoral Researcher focusing on the effect of drugs on the metabolism of a Shigella infected host cell and how this knowledge can be exploited to control Shigella growth
Dr Olga Timoshenko, Postdoctoral Researcher performing RNAi screens to identify host metabolic genes important for Shigella flexneri growth inside the human cells
Dr Julien Limenitakis, Postdoctoral Researcher working with dedicated software for integration of Omics data together with in silico models of Shigella flexneri infected host cells in order to refine the computer-based model and identify key metabolic nodes for Shigella growth. The cell biology and physiology of the host cell are also being assessed experimentally providing additional parameters to refine the model.
Petra Tienz, Technical Assistant
Beatrice Müller, Technical Assistant
Dr Rachel Benzies, Project Co-ordinator supporting the project team in Basel and coordination across BattleX.